Metabolite secretion, Fe(3+)-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869.
Identifieur interne : 000970 ( Main/Exploration ); précédent : 000969; suivant : 000971Metabolite secretion, Fe(3+)-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869.
Auteurs : André Aguiar [Brésil] ; Daniela Gavioli [Brésil] ; André Ferraz [Brésil]Source :
- Fungal biology [ 1878-6146 ] ; 2014.
Descripteurs français
- KwdFr :
- Bois (composition chimique), Bois (microbiologie), Bois (métabolisme), Composés du fer III (métabolisme), Facteurs temps (MeSH), Fermentation (MeSH), Hydrolases (analyse), Mélanges complexes (isolement et purification), Oxydoréduction (MeSH), Pinus taeda (microbiologie), Polyosides (analyse), Trametes (croissance et développement), Trametes (métabolisme).
- MESH :
- analyse : Hydrolases, Polyosides.
- composition chimique : Bois.
- croissance et développement : Trametes.
- isolement et purification : Mélanges complexes.
- microbiologie : Bois, Pinus taeda.
- métabolisme : Bois, Composés du fer III, Trametes.
- Facteurs temps, Fermentation, Oxydoréduction.
English descriptors
- KwdEn :
- Complex Mixtures (isolation & purification), Fermentation (MeSH), Ferric Compounds (metabolism), Hydrolases (analysis), Oxidation-Reduction (MeSH), Pinus taeda (microbiology), Polysaccharides (analysis), Time Factors (MeSH), Trametes (growth & development), Trametes (metabolism), Wood (chemistry), Wood (metabolism), Wood (microbiology).
- MESH :
- chemical , analysis : Hydrolases, Polysaccharides.
- chemical , isolation & purification : Complex Mixtures.
- chemical , metabolism : Ferric Compounds.
- chemistry : Wood.
- growth & development : Trametes.
- metabolism : Trametes, Wood.
- microbiology : Pinus taeda, Wood.
- Fermentation, Oxidation-Reduction, Time Factors.
Abstract
Trametes versicolor is a promising white-rot fungus for the biological pretreatment of lignocellulosic biomass. In the present work, T. versicolor ATCC 20869 was grown on Pinus taeda wood chips under solid-state fermentation conditions to examine the wood-degrading mechanisms employed by this fungus. Samples that were subjected to fungal pretreatment for one-, two- and four-week periods were investigated. The average mass loss ranged from 5 % to 8 % (m m(-)(1)). The polysaccharides were preferentially degraded: hemicellulose and glucan losses reached 13.4 % and 6.9 % (m m(-)(1)) after four weeks of cultivation, respectively. Crude enzyme extracts were obtained and assayed using specific substrates and their enzymatic activities were measured. Xylanases were the predominant enzymes, while cellobiohydrolase activities were marginally detected. Endoglucanase activity, β-glucosidase activity, and wood glucan losses increased up to the second week of biodegradation and remained constant after that time. Although no lignin-degrading enzyme activity was detected, the lignin loss reached 7.5 % (m m(-)(1)). Soluble oxalic acid was detected in trace quantities. After the first week of biodegradation, the Fe(3+)-reducing activity steadily increased with time, but the activity levels were always lower than those observed in the undecayed wood. The progressive wood polymer degradation appeared related to the secretion of hydrolytic enzymes, as well as to Fe(3+)-reducing activity, which was restored in the cultures after the first week of biodegradation.
DOI: 10.1016/j.funbio.2014.08.004
PubMed: 25442296
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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In the present work, T. versicolor ATCC 20869 was grown on Pinus taeda wood chips under solid-state fermentation conditions to examine the wood-degrading mechanisms employed by this fungus. Samples that were subjected to fungal pretreatment for one-, two- and four-week periods were investigated. The average mass loss ranged from 5 % to 8 % (m m(-)(1)). The polysaccharides were preferentially degraded: hemicellulose and glucan losses reached 13.4 % and 6.9 % (m m(-)(1)) after four weeks of cultivation, respectively. Crude enzyme extracts were obtained and assayed using specific substrates and their enzymatic activities were measured. Xylanases were the predominant enzymes, while cellobiohydrolase activities were marginally detected. Endoglucanase activity, β-glucosidase activity, and wood glucan losses increased up to the second week of biodegradation and remained constant after that time. Although no lignin-degrading enzyme activity was detected, the lignin loss reached 7.5 % (m m(-)(1)). Soluble oxalic acid was detected in trace quantities. After the first week of biodegradation, the Fe(3+)-reducing activity steadily increased with time, but the activity levels were always lower than those observed in the undecayed wood. The progressive wood polymer degradation appeared related to the secretion of hydrolytic enzymes, as well as to Fe(3+)-reducing activity, which was restored in the cultures after the first week of biodegradation. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25442296</PMID><DateCompleted><Year>2015</Year><Month>07</Month><Day>08</Day></DateCompleted><DateRevised><Year>2014</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>118</Volume><Issue>11</Issue><PubDate><Year>2014</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Metabolite secretion, Fe(3+)-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869.</ArticleTitle><Pagination><MedlinePgn>935-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2014.08.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1878-6146(14)00134-2</ELocationID><Abstract><AbstractText>Trametes versicolor is a promising white-rot fungus for the biological pretreatment of lignocellulosic biomass. In the present work, T. versicolor ATCC 20869 was grown on Pinus taeda wood chips under solid-state fermentation conditions to examine the wood-degrading mechanisms employed by this fungus. Samples that were subjected to fungal pretreatment for one-, two- and four-week periods were investigated. The average mass loss ranged from 5 % to 8 % (m m(-)(1)). The polysaccharides were preferentially degraded: hemicellulose and glucan losses reached 13.4 % and 6.9 % (m m(-)(1)) after four weeks of cultivation, respectively. Crude enzyme extracts were obtained and assayed using specific substrates and their enzymatic activities were measured. Xylanases were the predominant enzymes, while cellobiohydrolase activities were marginally detected. Endoglucanase activity, β-glucosidase activity, and wood glucan losses increased up to the second week of biodegradation and remained constant after that time. Although no lignin-degrading enzyme activity was detected, the lignin loss reached 7.5 % (m m(-)(1)). Soluble oxalic acid was detected in trace quantities. After the first week of biodegradation, the Fe(3+)-reducing activity steadily increased with time, but the activity levels were always lower than those observed in the undecayed wood. The progressive wood polymer degradation appeared related to the secretion of hydrolytic enzymes, as well as to Fe(3+)-reducing activity, which was restored in the cultures after the first week of biodegradation. </AbstractText><CopyrightInformation>Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aguiar</LastName><ForeName>André</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Instituto de Recursos Naturais, Universidade Federal de Itajubá, CP 50, 37500-903 Itajubá, MG, Brazil. Electronic address: aguiar@unifei.edu.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gavioli</LastName><ForeName>Daniela</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, SP, Brazil. Electronic address: dandagavioli@yahoo.com.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferraz</LastName><ForeName>André</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, SP, Brazil. 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